Coliform Analytical Methods and Data Application in Wastewater and Recreational Waters

The three most common analytical techniques for coliform analysis are Membrane Filtration, Multiple Tube Fermentation and Defined Substrate. This webcast will cover basic aseptic technique, which is critical to the success of any microbiology method, as well as the ins and outs of all three of these types of microbiological analyses for coliform bacteria. Coliform results are used as an indicator of effective disinfection as a key component of wastewater treatment, as well as an indicator of recreational waterway health.  The theory and appropriate uses will also be discussed.  In addition the quality control associated with each of these techniques will be discussed.  The application of coliform results in making compliance and recreational waterway health decisions will also be covered, including the importance of timely results being provided to data users.

  • This webcast will cover the three most common analytical techniques used for coliform analysis and the key components of aseptic technique, a key component of all of coliform analyses.
  • Theory, appropriate use and quality control for coliform methods will be covered
  • Application of data in making decisions regarding the effectiveness of disinfection in the wastewater treatment process and as an indicator of recreational waterway health will be discussed.


Anthony Russo, Biological Services Section Chief, Pennsylvania Department of Environmental Protection

Akin Babatola, MS Molecular Biology, Laboratory & Environmental Compliance Manager, City of Santa Cruz, CA

Gil Dichter, Worldwide Technical Support Manager, Water, IDEXX Laboratories

Stacie Metzler (Moderator), Laboratory Division Chief, HRSD

This webcast will be presented in cooperation with the Association of Public Health Laboratories (APHL).

There will be up to 2.0 Professional Development Hours (PDHs) offered for this webcast. Please check with your state accreditation agency to determine if you qualify. The PDH instructions will be sent to all attendees 24 hours after the webcast has ended.